E3S Web Conf.
Volume 78, 20192018 International Seminar on Food Safety and Environmental Engineering (FSEE 2018)
|Number of page(s)||6|
|Section||Food Safety, Extraction and Food Engineering|
|Published online||15 January 2019|
Catalytic synthesis of β-sitosterol linolenate by Pickering emulsion-immobilized lipase
School of Biological Engineering, Tianjin Modern Vocational Technology College, Tianjin, 300350, China
* Corresponding author: firstname.lastname@example.org (Lihui Wang)
In order to promote the application of phytosterol ester, a new kind of catalyst was developed on the basis of traditional enzymatic synthesis method. Pickering emulsion catalyst (CRL@PE) with two-phase structure was constructed using isoctane and Candida rugosa lipase and employing nanotube as emulsifier. Then the CRL@PE was used to catalyze the synthesis of β-sitosterol linolenic acid ester with β-sitosterol and α-linolenic acid as substrates. The effects of temperature, enzyme dosage, linolenic acid concentration and reaction time on the conversion rate of phytosterols were studied by single factor experiment and central composite design of response surface methodology. The optimal process parameters were obtained as follows: temperature of 29.45 oC, lipase dossage of 10.97 U, linolenic acid concentration of 42.35 mmol/L, reaction time of 350.7 min, and phytosterol conversion rate was 96.8±0.7% at these conditions. After reuse of CRL@PE for 10 times, the phytosterol conversion rate was 96.86%, showing that the catalyst had good stability.
© The Authors, published by EDP Sciences, 2019
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Current usage metrics show cumulative count of Article Views (full-text article views including HTML views, PDF and ePub downloads, according to the available data) and Abstracts Views on Vision4Press platform.
Data correspond to usage on the plateform after 2015. The current usage metrics is available 48-96 hours after online publication and is updated daily on week days.
Initial download of the metrics may take a while.