E3S Web Conf.
Volume 145, 20202019 International Academic Exchange Conference on Science and Technology Innovation (IAECST 2019)
|Number of page(s)||4|
|Section||International Conference on Biotechnology and Food Science|
|Published online||06 February 2020|
Daily accumulation of melatonin and gene expression in chinese cherry
1 Collega of Horticulture, Sichuan Agricultural University, Chengdu, Sichuan, China
2 Science Technology & Agriculture Livestock Bureau of Wenchuan county, Aba Tibetan and Qiang Autonomous Prefecture, Sichuan, 623000, China
3 Insitute of pomology and Olericulture, Sichuan Agricultural University, Chengdu, Sichuan, China
a Corresponding author’s e-mail: firstname.lastname@example.org
To explore the accumulation characteristics of melatonin and underline mechanism in leaves of chinese cherry, the daily changes of melatonin content and the expression of synthetic genes were determined. The results showed that melatonin content increased gradually during the day and midnight, reaching the peak at 2:00 a.m, and then began to decrease, indicating that light regulate the synthesis of melatonin in plants. The expression profile of 5 melatonin synthesis genes was investigated by qRT-PCR, among them the mRNA transcript of PacSNAT, PacASMT and PacT5H2 increased and then decreased with time prolonging, in accordance with changes of melatonin content, indicating their key role in melatonin synthesis.
© The Authors, published by EDP Sciences 2020
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