Study of superoxiddismutase activity dynamics in rat skeletal muscle tissue under mechanical influence on hemato-ophthalmic barrier

¹ Kazan State Academy of Veterinary Medicine named after N.E. Bauman, 420074, st. Siberian tract, 35, Kazan, Republic of Tatarstan, Russia
² Samara State University of Railways, 443077, Svobody st., 2В, Samara, Russia
³ Private institution educational organization of higher education "Medical University "Reaviz", 443099, st. Chapaevskaya, 227, Samara, Russia
⁴ Samara State Medical University" of the Ministry of Health of the Russian Federation, 443099, st. Chapaevskaya, 89, Samara, Russia

^* Corresponding author: casiopeya13@mail.ru

Abstract

Hemato-ophthalmic barrier is one of the mechanisms of body resistance. One of the complications of mechanical trauma of the eye and violation of the hemato-ophthalmic barrier is the emergence of oxidative stress on the background of the general inflammatory process. Normally, oxidative stress in the skeletal muscle tissue is not a damaging agent, but when intensified by other factors, it promotes pathological changes in the body. Objective: to study the dynamics of superoxiddismutase (SOD) activity in rat skeletal muscle tissue under oxidative stress caused by mechanical action on the hemato-ophthalmic barrier. Materials and methods: The study was carried out on pedigree matured male rats in the amount of 150 pieces. The activity of SOD in skeletal muscle tissue was studied before the experiment, as well as on the 1st, 3rd, 5th, 7th and 14th day of the experiment using the standard technique of V.S. Gurevich. The obtained digital material was subjected to statistical processing by means of non-parametric statistical analysis. Conclusion: SOD activity in rat skeletal muscle tissue under oxidative stress caused by mechanical action on hemato-ophthalmic barrier is most effectively stabilized in standard therapy of mechanical eye injury with the addition of quercetin in the form of injections.