E3S Web Conf.
Volume 328, 2021International Conference on Science and Technology (ICST 2021)
|Number of page(s)||7|
|Section||Natural Science, Natural Resources Management, Bio Technology, Microbiology|
|Published online||06 December 2021|
Molecular Identification of Pathogenic Bacteria in Kantong Semar Plants (Nepenthes Gracillis) Based on Mitochondrial 16S rRNA Gene
Biology Department, Universitas Negeri Surabaya
* Corresponding author: email@example.com
Nepenthes is a protected and unique carnivorous plant because it has a fluid-filled pouch at the end of the leaf that serves as an insect trap. The existence of Nepenthes gracillis in ecosystems is endangered, one of the reasons is caused by the attack of pathogenic bacteria, so it is necessary to identify pathogenic bacteria that attack N. gracillis as an effort to handle. The purpose of this study was to identify pathogenic bacteria in N. gracillis using 16S rRNA gene sequencing results. This study aims to identify the 16S rRNA sequence and phylogenetic of bacteria attached in N. Gracillis leaves, and the identification was confirmed using molecular data. The construction of phylogenetic topology was made based on ML and NJ method using Kimura-2 parameter model. Subsequently, molecular characterization and identification was conducted based on 720 bp of 16S rRNA gene similarity, sequence variation, genetic distance, and phylogenetic topology. According to the results, the bacteria species attached in N. Gracillis identified as Lelliottia nimipressuralis, and closely related with Erwinia aphidicola. Genetic distance between sample and related species was 0,0173, with 98,26% similarities, then strongly 100 bootstrap analyses. Thus, this study provides more information for Lelliottia nimipressuralis phylogenetic studies using different marker.
Key words: Nepenthes gracilis / Pathogenic bacteria / Mitochondrial gene
© The Authors, published by EDP Sciences, 2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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