Issue |
E3S Web Conf.
Volume 431, 2023
XI International Scientific and Practical Conference Innovative Technologies in Environmental Science and Education (ITSE-2023)
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Article Number | 01042 | |
Number of page(s) | 5 | |
Section | Agricultural Innovation Systems | |
DOI | https://doi.org/10.1051/e3sconf/202343101042 | |
Published online | 13 October 2023 |
Investigation of ice particle formation points during fish sperm cryopreservation
1 Federal Research Centre The Southern Scientific Centre of the Russian Academy of Sciences, 41, Chekhov str., 344006, Rostov-on-Don, Russia
2 Don State Technical University, 1 Gagarin square, 344002, Rostov-on-Don, Russia
* Corresponding author: reception@donstu.ru
This study was conducted in order to investigate of the points of formation of ice particles during cooling of fish sperm. The object of the study was spermatozoa of the Russian sturgeon (Acipenser gueldenstaedtii Brandt, 1833), hybrid Acipenser ruthenus × Huso huso, the spiny-tailed sturgeon Acipenser nudiventris Lovetsky, 1828, and the inconnu Stenodus leucichthys Güldenstädt, 1772. The growth of ice microparticles is formed at the perturbation temperature of the corresponding aqueous suspension, with the concentration of dissolved substances increasing in the non-freezing phase. The process of ice formation and the increase concentration of the remaining liquid continues as it cools to form amorphous (glassy) ice. It is interesting to bring the mass of ice with a further decrease in temperature to (-196) ° C. Cooling causes compression of the formed ice crystals.
© The Authors, published by EDP Sciences, 2023
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