| Issue |
E3S Web Conf.
Volume 282, 2021
International Conference “Ensuring Food Security in the Context of the COVID-19 Pandemic” (EFSC2021)
|
|
|---|---|---|
| Article Number | 04009 | |
| Number of page(s) | 7 | |
| Section | Development of Production of Compound Feeds, Feed Additives for Animals, Medicines for Veterinary Use, Mineral Additives | |
| DOI | https://doi.org/10.1051/e3sconf/202128204009 | |
| Published online | 05 July 2021 | |
Development of polyvalent toxoid Clostridium perfringens against anaerobic enterotoxaemia in young cattle and small ruminants
1 Institute for Problems Biological Safety and Biotechnology, Tajik Academy of Agricultural Sciences, Dushanbe, Republic of Tajikistan
2 Tajik National University, Dushanbe, Republic of Tajikistan
* Corresponding author: orzugul@mail.ru
The authors of the article presents the results of the development of a polyvalent toxoid from C.perfringens strains against anaerobic enterotoxaemia of young cattle and small ruminants and an assessment of its effectiveness. It is presented the data on the selection of the recipe composition of the nutrient medium, the cultivation of industrial strains, the process of drug preparation, the selection of the optimal ratio of components and the method of controlling the effectiveness. Sequential cultivation of industrial strains of C.perfringens was carried out by cultivating them in flasks, bottles and bioreactors in the nutrient medium developed by the authors, consisting of: 12.0 g/l - liver extract; 25.0 g/l - casein-peptone; 3.2g/l –K2HPO4; 1.8 g/l – KH2PO4; 0.5 g/l – MgSO4. The development of the drug included the selection of optimal and balanced ratios of C. perfringens type A toxoids - 15 IU/ml, C.perfringens type B - 20 IU/ml, C.perfringens type C - 30 IU/ml, C. perfringens type D - 30 IU/ml, which ensured 100% protection of the immunized animals. Studies on laboratory and farm animals revealed the harmlessness, areactogenicity of the drug, which stimulated the development of immunity and resistance to the toxic effects of all strains of C.perfringens.
© The Authors, published by EDP Sciences, 2021
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