| Issue |
E3S Web Conf.
Volume 292, 2021
2021 2nd International Conference on New Energy Technology and Industrial Development (NETID 2021)
|
|
|---|---|---|
| Article Number | 03070 | |
| Number of page(s) | 4 | |
| Section | Environmental Sustainable Development and Industrial Transformation | |
| DOI | https://doi.org/10.1051/e3sconf/202129203070 | |
| Published online | 09 September 2021 | |
Initial Characterization and Expression Pattern Analysis of Tobacco (Nicotiana Tabacum) GMP Synthase Gene
1 College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China
2 College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China
3 Research and Development Center, China Tobacco Yunnan Industrial Co.,Ltd, Kunming 650106, China
* Corresponding author: zhaozx0801@163.com
The complete mRNA sequence of one tobacco (nicotiana tabacum) gene—guanosine monophosphate (GMP)synthase, was amplified using the rapid amplification of cDNA ends methods. The full-length tobacco GMP synthase gene mRNA was 2,127bp containing a 1,617 bp open reading frame, which encodes a protein of 538 amino acids. Sequence analysis revealed that the GMP synthase of tobacco shares high homology with the GMP synthase of wood tobacco(99%), nicotiana attenuata(99%), nicotiana tomentosiformis(99%), potato(92%), Lycopersicon pennellii(92%), lycopersicon esculentum(92%), capsicum annuum(91%), capsicum chinense(91%) and capsicum baccatum(90%). BLAST analysis within the tobacco high throughout genomic sequences database revealed that this gene has 5 introns and 6 exons. Results also showed that tobacco GMP synthase gene has a closer genetic relationship with the GMP synthase gene of wood tobacco. Tissue expression profile analysis revealed that the tobacco GMP synthase gene was highly expressed in leaf, but moderately expressed in root, flower and stem. Our experiment established the foundation for further research on this tobacco gene.
© The Authors, published by EDP Sciences, 2021
This is an Open Access article distributed under the terms of the Creative Commons Attribution License 4.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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