| Issue |
E3S Web Conf.
Volume 292, 2021
2021 2nd International Conference on New Energy Technology and Industrial Development (NETID 2021)
|
|
|---|---|---|
| Article Number | 03094 | |
| Number of page(s) | 4 | |
| Section | Environmental Sustainable Development and Industrial Transformation | |
| DOI | https://doi.org/10.1051/e3sconf/202129203094 | |
| Published online | 09 September 2021 | |
Initial Characterization and Expression Pattern Analysis of Tobacco (Nicotiana Tabacum) HMGS Gene
1 College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China
2 College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China
3 Research and Development Center, China Tobacco Yunnan Industrial Co.,Ltd, Kunming 650106, China
* Corresponding author: zhaozx0801@163.com
3-hydroxy-3-methylglutaryl coenzyme A synthase (HMGS) is a member of condensing enzymes that catalyze a Claisen-like condensation reaction.The tobacco (nicotiana tabacum) HMGS gene was firstly characterized using the rapid amplification of cDNA ends methods based on one tobacco EST. The full-length tobacco HMGS gene mRNA was 1,773bp containing a 1389 bp open reading frame, which encodes a protein of 462 amino acids. Sequence analysis revealed that the HMGS of tobacco shares high homology with the HMGS of nicotiana tomentosiformis (96%), nicotiana attenuata (95%), Nicotiana sylvestris (95%), nicotiana benthamiana(94%), solanum lycopersicum(94%), solanum tuberosum(93%) and withania somnifera(93%). Results also showed that tobacco HMGS gene has a closer genetic relationship with the HMGS gene of withania somnifera. Tissue expression profile analysis revealed that the tobacco HMGS gene was highly expressed in flower, but moderately expressed in leaf and stem, and weakly expressed in root. Our experiment established the foundation for further research on this tobacco gene.
© The Authors, published by EDP Sciences, 2021
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