E3S Web Conf.
Volume 335, 2022The 2nd International Conference on Environmentally Sustainable Animal Industry (The 2nd ICESAI 2021)
|Number of page(s)||5|
|Published online||14 January 2022|
Monomorphic allele rs109231213 in 3’UTR PLAG1 gene in purebred of Bali cattle (Bos javanicus)
1 Research Center for Biology, National Research and Innovation Agency (BRIN), Bogor 16911, Indonesia
2 Division of Animal and Dairy Science, Department of Animal Science, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 34134, Korea
3 Indonesian Research Institute for Animal Production, Bogor 16720, Indonesia
4 Faculty of Animal Science, Bogor Agricultural University, Bogor 16680, Indonesia
* Corresponding author: firstname.lastname@example.org
The mutation rs109231213 that is located in 3’UTR of PLAG1 gene is associated with the growth and body weight in several Bos taurus and Bos indicus breeds. This study aimed to identify SNP rs109231213 in Bali cattle (Bos javanicus). The study used 41 samples of Bali cattle. The PLAG1 gene polymorphism was analyzed using PCR and direct sequencing methods. PCR pimers were 5’- TTGCACAGAATCAGTGTGTC-3’ and 5’- AGCCTAACGTGGATCTATGG-3’. The results showed that primers successfully amplified the 331 bp fragment at annealing 60°C that contained rs109231213. SNP was monomorphic in Bali cattle with one allele (G). This study concludes that rs109231213 in 3’UTR of PLAG1 gene can be used as specific marker in purebred of Bali cattle that have never been crossed with Bos taurus and Bos indicus.
© The Authors, published by EDP Sciences, 2022
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