E3S Web Conf.
Volume 335, 2022The 2nd International Conference on Environmentally Sustainable Animal Industry (The 2nd ICESAI 2021)
|Number of page(s)||5|
|Published online||14 January 2022|
Identification of 19-bp indel of the Pleomorphic Adenoma Gene 1 in Bali cattle population
1 Department of Animal Science, Sebelas Maret University, Surakarta 57126, Indonesia
2 Research Center for Biotechnology, Research Organization for Life Sciences, National Research and Innovation Agency (BRIN), Cibinong 16911, Indonesia
3 Assessment Institute of Agricultural Technology-Central Java, Semarang 50552, Indonesia
* Corresponding author: email@example.com
Pleomorphic adenoma gene 1 (PLAG1) is a zinc finger transcription factor gene located on bovine chromosome 14 (BTA14) affecting body size and reproduction traits in cattle. The objective of this study was to identify 19-bp indel of the PLAG1 gene in Bali cattle population. A total of 96 blood samples of Bali Cattle were collected from Balai Pembibitan Ternak Unggul dan Hijauan Pakan Ternak (BPTU-HPT) Denpasar. Genomic DNA was extracted from blood samples and used to detect 19-bp indel of the PLAG1 gene using following primer pair 5’-TCCGAACAACAGGTGAGGGAGAAAT-3’ and 5’-CCACTTCAGG-GGTGCTCTAGGTTTG-3’. The polymerase chain reaction (PCR) products using DNA pool samples were sequenced to validate the PCR product and to find out novel polymorphism in Bali cattle population. The result showed that there was no variation found in Bali cattle population based on 19-bp indel of the PLAG1 gene, which is indicated by 123 bp DNA band. However, sequence analysis of the PLAG1 gene resulted in a novel single nucleotide polymorphism (SNP) at nucleotide number 32235 of the PLAG1 gene that changed guanine (G) to adenine (A). This novel SNP could be furthermore genotyped and it might be a potential candidate marker for body size and reproduction traits in Bali cattle.
© The Authors, published by EDP Sciences, 2022
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