Kinetics study of bacterial cellulose production by Acetobacter xylinum FNCC 0001 with variation of carbon sources

Department of Food Science and Technology, Universitas Sebelas Maret, Ir Sutami Street 36A Surakarta 57126, Indonesia

^* Corresponding author: adhit.sanjaya@staff.uns.ac.id

Abstract

Glucose and fructose (2.0% (w/v)) were used as the carbon sources and peptone was used as nitrogen source (1.5% (w/v)) in order to determine the kinetics study and yield of bacterial cellulose production. Coconut water was prepared as the fermentation medium. Data of the fermentation rate was observed at 19 points of certain time during 7 days of fermentation process. The number of cell biomass was calculated using cell dried weight method, total plate count method and optical density method which was observed at 660 nm. The remaining media substrate was analyzed using the DNS method, while the BC yield was determined by the gravimetric method. The results showed that the best values of μ, Td, Rx, Rs, Rp, Yp/s, Yp/x were achieved by fructose, while the best n and Yx/s value were achieved by glucose. The corresponding values were 0,1141/h, 6,0737 h, 0,1141 g/L/h, 0,0635 g/L/h, 0,3015 g/L/h, 0,317 g/g, 0,5927 g/g 1,3631 times and 0,0983 g/g respectively. The highest BC yield at the end of fermentation process was achieved by glucose carbon at 5.83 g/L, followed by fructose at 4.91 g/L.