Establishment of hydrolysates from enzymatic plant and fish protein by-products into fish nutrition

¹ Russian State Agrarian University – Moscow Timiryazev Agricultural Academy, 49, Timiryazevskaya st., Moscow, 127434, Russia
² Republican Daughter Unitary Enterprise "Fish Industry Institute" of the Republican Unitary Enterprise “Scientific and Practical Center of the National Academy of Sciences of Belarus for Animal Husbandry”, 22, Stebeneva str., Minsk, 220024, Belarus

^* Corresponding author: d.aleshin@rgau-msha.ru

Abstract

The aim of this research was to scientifically prove the technology for producing plant-fish hydrolysates from sunflower meal and fish waste (carp processing) and to develop scientifically based recipes for protein hydrolysates and feed using it for carp of different ages. Compositions from fish waste and different amounts of sunflower meal have been developed, the chemical composition of the compositions and the digestibility of protein by carp have been studied. It was distinguished that the best characteristics when studing the nutritional value and digestibility of crude protein have the protein hydrolysates of fish waste and sunflower meal, taken in a ratio of 1 to 2. Accordingly, in this case, a high content of dry matter was noted 65.57 ± 0.18%), crude protein 41.92±0.72%, crude fat 2.74±0.12% and low crude fiber 14.34±0.10%, and the apparent protein digestibility coefficient was 78.8%. The compiled enzyme compositions for enzymatic hydrolysis are optimal at a temperature of 50°C and the proportion of the enzyme composition for fermentation in an amount of 5%, containing enzyme preparations: Protozyme - 40%, Cellulase - 35%, Lipase - 10% and Amylorizin - 15%.