E3S Web Conf.
Volume 254, 2021International Scientific and Practical Conference “Fundamental and Applied Research in Biology and Agriculture: Current Issues, Achievements and Innovations” (FARBA 2021)
|Number of page(s)||8|
|Section||The Role of Biotechnology in Obtaining Pure Virus-free Material|
|Published online||05 May 2021|
Optimization of individual elements of clonal micro-propagation of fruit and berry crops in the production system of healthy planting material
FSBSI All-Russian Research Institute of Fruit Crop Breeding, Oryol region, Russia
* Corresponding author: email@example.com
Clonal micro-propagation is of particular importance in the transition to virus-free gardening. The problems of in vitro cultivation are closely related to mineral nutrition, presence of growth hormones and varietal features of plants. The purpose of the research was to study the patterns of responses of the studied genotypes of fruit and berry crops to the effect of various nutrient media. The objects of the study were varieties of garden strawberries, blackberries, pears and apple trees. Single shoots were planted on a modified medium of MS, Boxus, QL, B5, Pierik, Ly de Fossard, Fardzinova. The number of developed buds per explant, the height of micro-shoots, the general appearance and development of micro-plants were taken into account. It was found that exclusion of ammonium nitrate (NH4NO3) and replacement of calcium chloride (CaCl2) with calcium nitrate (Ca (NO3)2) in the Boxus nutrient medium in strawberry provided obtaining optimally developed plants. For blackberries, the best results of proliferation and growth of shoots were obtained on the growing medium of Ly de Fossard, in pear varieties - on the Ly de Fossard medium. For most of the studied apple tree varieties, the most favorable medium was QL, containing 2.0 mg/l of 6-BAP.
© The Authors, published by EDP Sciences, 2021
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