| Issue |
E3S Web Conf.
Volume 254, 2021
International Scientific and Practical Conference “Fundamental and Applied Research in Biology and Agriculture: Current Issues, Achievements and Innovations” (FARBA 2021)
|
|
|---|---|---|
| Article Number | 04003 | |
| Number of page(s) | 8 | |
| Section | The Role of Biotechnology in Obtaining Pure Virus-free Material | |
| DOI | https://doi.org/10.1051/e3sconf/202125404003 | |
| Published online | 05 May 2021 | |
Optimization of the technology of clonal micro-propagation of cherry varieties and clonal rootstocks at the initial stages of in vitro cultivation
FSBEI HE “OSU n.a. I.S. Turgenev”, Orel, Russia
* Corresponding author: ostrikova_ov@mail.ru
The article presents the results of studying some components of the in vitro technology of clonal rootstocks and common cherry varieties, which allow to increase the efficiency of its application to the tested cherry varieties and clonal rootstocks. Differences in the viability and intensity of the development of clonal cherry rootstocks and varieties explants depending on the timing of their introduction into the crop, the characteristics of genotypes and the composition of nutrient media were established. The influence of nutrient medium composition, content and ratio of growth regulators and genotype in it on the multiplication coefficient of cherry varieties and clonal rootstocks was revealed. The optimal calendar dates for the introduction of cherry varieties and clonal rootstocks explants into the crop in vitro for the autumn-winter-spring period are established. The optimal composition of the culture medium for the initial stages of clonal micropropagation is established. The reproductive potential of some clonal rootstocks and cherry varieties in crop in vitro was revealed.
© The Authors, published by EDP Sciences, 2021
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